The characteristics of proteome and metabolome associated with contrasting sperm motility in goat seminal plasma

Abstract Sperm motility is an index tightly associated with male fertility. A close relationship between seminal plasma and sperm has been confirmed. This study was to assess the protein metabolite profiles of obtained from adult goats high or low using proteomic metabolomic strategies. In total, 2098 proteins were found. 449 differentially abundant (DAPs) identified, 175 DAPs enriched in group. The primarily exist cytoplasma extra-cellular portion. Gene Ontology enrichment analysis demonstrated main functional roles these regulating biological process, metabolic process organic substances, cellular-metabolic primary-metabolic nitrogen compounds, etc. Additionally, Kyoto-Encyclopedia Genes Genomes (KEGG) revealed that involved phosphatidylinositol signaling system, salivary secretion, proteasome, apoptosis, mitophagy-animal, Aided by parallel reaction monitoring technology, abundance changing pattern 19 selected consistent corresponding TMT. total 4603 metabolites identified plasma. 1857 differential found group group, 999 up-regulated KEGG primary involvement synthetic activities. conclusion, we first established proteome metabolome databank goat plasma, detecting some which may affect motility. will be valuable for understanding mechanisms leading poor